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wild type m pneumoniae strain m129 b7  (ATCC)


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    Structured Review

    ATCC wild type m pneumoniae strain m129 b7
    Wild Type M Pneumoniae Strain M129 B7, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 525 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wild+type+m+pneumoniae+strain+m129/B-7/bio_rxiv__64898__2026__04__12__717927-147-15-20
    Average 96 stars, based on 525 article reviews
    wild type m pneumoniae strain m129 b7 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: Engineered Mycoplasma pneumoniae targeting dual-species bacterial biofilms: a novel strategy against infections
    Article Snippet: Strains P. aeruginosa PAR10471 and S. aureus SAR10471 are clinical isolates from a patient suffering respiratory problems .Strains P. aeruginosa PAR10471 and S. aureus SAR10471 are clinical isolates from a patient suffering respiratory problems .. The attenuated strain CV8 derives from the wild-type M. pneumoniae strain M129 (ATCC 29342, subtype 1), in which genes mpn133 and mpn372 were deleted, and the mpn051 gene was replaced by the gpsA gene from Mycoplasma penetrans .. M. pneumoniae strains were cultured in T75 flasks (VWR) with 25 ml of modified Hayflick medium (here called Hayflick) and incubated at 37 °C in a 5% CO 2 supplement until the late exponential phase.M. pneumoniae strains were cultured in T75 flasks (VWR) with 25 ml of modified Hayflick medium (here called Hayflick) and incubated at 37 °C in a 5% CO 2 supplement until the late exponential phase.

    Expressing:

    Article Title: Engineered Mycoplasma pneumoniae targeting dual-species bacterial biofilms: a novel strategy against infections
    Article Snippet: Strains P. aeruginosa PAR10471 and S. aureus SAR10471 are clinical isolates from a patient suffering respiratory problems .Strains P. aeruginosa PAR10471 and S. aureus SAR10471 are clinical isolates from a patient suffering respiratory problems .. The attenuated strain CV8 derives from the wild-type M. pneumoniae strain M129 (ATCC 29342, subtype 1), in which genes mpn133 and mpn372 were deleted, and the mpn051 gene was replaced by the gpsA gene from Mycoplasma penetrans .. M. pneumoniae strains were cultured in T75 flasks (VWR) with 25 ml of modified Hayflick medium (here called Hayflick) and incubated at 37 °C in a 5% CO 2 supplement until the late exponential phase.M. pneumoniae strains were cultured in T75 flasks (VWR) with 25 ml of modified Hayflick medium (here called Hayflick) and incubated at 37 °C in a 5% CO 2 supplement until the late exponential phase.

    Dissolution:

    Article Title: Engineered Mycoplasma pneumoniae targeting dual-species bacterial biofilms: a novel strategy against infections
    Article Snippet: Strains P. aeruginosa PAR10471 and S. aureus SAR10471 are clinical isolates from a patient suffering respiratory problems .Strains P. aeruginosa PAR10471 and S. aureus SAR10471 are clinical isolates from a patient suffering respiratory problems .. The attenuated strain CV8 derives from the wild-type M. pneumoniae strain M129 (ATCC 29342, subtype 1), in which genes mpn133 and mpn372 were deleted, and the mpn051 gene was replaced by the gpsA gene from Mycoplasma penetrans .. M. pneumoniae strains were cultured in T75 flasks (VWR) with 25 ml of modified Hayflick medium (here called Hayflick) and incubated at 37 °C in a 5% CO 2 supplement until the late exponential phase.M. pneumoniae strains were cultured in T75 flasks (VWR) with 25 ml of modified Hayflick medium (here called Hayflick) and incubated at 37 °C in a 5% CO 2 supplement until the late exponential phase.

    In Vitro:

    Article Title: Engineered Mycoplasma pneumoniae targeting dual-species bacterial biofilms: a novel strategy against infections
    Article Snippet: Strains P. aeruginosa PAR10471 and S. aureus SAR10471 are clinical isolates from a patient suffering respiratory problems .Strains P. aeruginosa PAR10471 and S. aureus SAR10471 are clinical isolates from a patient suffering respiratory problems .. The attenuated strain CV8 derives from the wild-type M. pneumoniae strain M129 (ATCC 29342, subtype 1), in which genes mpn133 and mpn372 were deleted, and the mpn051 gene was replaced by the gpsA gene from Mycoplasma penetrans .. M. pneumoniae strains were cultured in T75 flasks (VWR) with 25 ml of modified Hayflick medium (here called Hayflick) and incubated at 37 °C in a 5% CO 2 supplement until the late exponential phase.M. pneumoniae strains were cultured in T75 flasks (VWR) with 25 ml of modified Hayflick medium (here called Hayflick) and incubated at 37 °C in a 5% CO 2 supplement until the late exponential phase.

    Crystal Violet Assay:

    Article Title: Engineered Mycoplasma pneumoniae targeting dual-species bacterial biofilms: a novel strategy against infections
    Article Snippet: Strains P. aeruginosa PAR10471 and S. aureus SAR10471 are clinical isolates from a patient suffering respiratory problems .Strains P. aeruginosa PAR10471 and S. aureus SAR10471 are clinical isolates from a patient suffering respiratory problems .. The attenuated strain CV8 derives from the wild-type M. pneumoniae strain M129 (ATCC 29342, subtype 1), in which genes mpn133 and mpn372 were deleted, and the mpn051 gene was replaced by the gpsA gene from Mycoplasma penetrans .. M. pneumoniae strains were cultured in T75 flasks (VWR) with 25 ml of modified Hayflick medium (here called Hayflick) and incubated at 37 °C in a 5% CO 2 supplement until the late exponential phase.M. pneumoniae strains were cultured in T75 flasks (VWR) with 25 ml of modified Hayflick medium (here called Hayflick) and incubated at 37 °C in a 5% CO 2 supplement until the late exponential phase.

    Comparison:

    Article Title: Engineered Mycoplasma pneumoniae targeting dual-species bacterial biofilms: a novel strategy against infections
    Article Snippet: Strains P. aeruginosa PAR10471 and S. aureus SAR10471 are clinical isolates from a patient suffering respiratory problems .Strains P. aeruginosa PAR10471 and S. aureus SAR10471 are clinical isolates from a patient suffering respiratory problems .. The attenuated strain CV8 derives from the wild-type M. pneumoniae strain M129 (ATCC 29342, subtype 1), in which genes mpn133 and mpn372 were deleted, and the mpn051 gene was replaced by the gpsA gene from Mycoplasma penetrans .. M. pneumoniae strains were cultured in T75 flasks (VWR) with 25 ml of modified Hayflick medium (here called Hayflick) and incubated at 37 °C in a 5% CO 2 supplement until the late exponential phase.M. pneumoniae strains were cultured in T75 flasks (VWR) with 25 ml of modified Hayflick medium (here called Hayflick) and incubated at 37 °C in a 5% CO 2 supplement until the late exponential phase.



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    wild type m pneumoniae strain m129 b7  (ATCC)
    96
    ATCC wild type m pneumoniae strain m129 b7
    Wild Type M Pneumoniae Strain M129 B7, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wild+type+m+pneumoniae+strain+m129/B-7/bio_rxiv__64898__2026__04__12__717927-147-15-20
    Average 96 stars, based on 1 article reviews
    wild type m pneumoniae strain m129 b7 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    wild type m pneumoniae strain m129  (ATCC)
    96
    ATCC wild type m pneumoniae strain m129
    a Graphical representation of the antibiofilm payloads secreted by M. <t>pneumoniae</t> strain CV8_HAD. b Western blot analysis of CV8_HAD lysate and supernatant (SN) showing expression of the payloads; the CV8 chassis and the loaded ribosomal protein RL7 were used as controls. c Representative images of crystal violet assays used to quantify S. aureus (strain Sa15981) or P. aeruginosa (strain PAO1) biofilms after treating with SN from CV8 strains. CV8_D expresses payload D only; CV8_HA expresses payloads H and A. d Plot showing the quantification of biofilm dissolution by CV8 strains SN treatments in vitro, using the crystal violet assay. Data are shown as the mean of three independent experiments ± SEM. *** p < 0.001, ****p < 0.0001 by one-way Anova followed by the post-hoc Tukey’s multiple comparison tests.
    Wild Type M Pneumoniae Strain M129, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wild+type+m+pneumoniae+strain+m129/Mycoplasmoides+pneumoniae+(Somerson+et+al%2E)+Gupta+et+al/pmc12623809-135-7-12
    Average 96 stars, based on 1 article reviews
    wild type m pneumoniae strain m129 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    m pneumoniae wild type strain m129  (ATCC)
    96
    ATCC m pneumoniae wild type strain m129
    a Graphical representation of the antibiofilm payloads secreted by M. <t>pneumoniae</t> strain CV8_HAD. b Western blot analysis of CV8_HAD lysate and supernatant (SN) showing expression of the payloads; the CV8 chassis and the loaded ribosomal protein RL7 were used as controls. c Representative images of crystal violet assays used to quantify S. aureus (strain Sa15981) or P. aeruginosa (strain PAO1) biofilms after treating with SN from CV8 strains. CV8_D expresses payload D only; CV8_HA expresses payloads H and A. d Plot showing the quantification of biofilm dissolution by CV8 strains SN treatments in vitro, using the crystal violet assay. Data are shown as the mean of three independent experiments ± SEM. *** p < 0.001, ****p < 0.0001 by one-way Anova followed by the post-hoc Tukey’s multiple comparison tests.
    M Pneumoniae Wild Type Strain M129, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wild+type+m+pneumoniae+strain+m129/Mycoplasmoides+pneumoniae+(Somerson+et+al%2E)+Gupta+et+al/10__1128_slash_iai__00268___09-55-1-6
    Average 96 stars, based on 1 article reviews
    m pneumoniae wild type strain m129 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    a Graphical representation of the antibiofilm payloads secreted by M. pneumoniae strain CV8_HAD. b Western blot analysis of CV8_HAD lysate and supernatant (SN) showing expression of the payloads; the CV8 chassis and the loaded ribosomal protein RL7 were used as controls. c Representative images of crystal violet assays used to quantify S. aureus (strain Sa15981) or P. aeruginosa (strain PAO1) biofilms after treating with SN from CV8 strains. CV8_D expresses payload D only; CV8_HA expresses payloads H and A. d Plot showing the quantification of biofilm dissolution by CV8 strains SN treatments in vitro, using the crystal violet assay. Data are shown as the mean of three independent experiments ± SEM. *** p < 0.001, ****p < 0.0001 by one-way Anova followed by the post-hoc Tukey’s multiple comparison tests.

    Journal: NPJ Biofilms and Microbiomes

    Article Title: Engineered Mycoplasma pneumoniae targeting dual-species bacterial biofilms: a novel strategy against infections

    doi: 10.1038/s41522-025-00835-2

    Figure Lengend Snippet: a Graphical representation of the antibiofilm payloads secreted by M. pneumoniae strain CV8_HAD. b Western blot analysis of CV8_HAD lysate and supernatant (SN) showing expression of the payloads; the CV8 chassis and the loaded ribosomal protein RL7 were used as controls. c Representative images of crystal violet assays used to quantify S. aureus (strain Sa15981) or P. aeruginosa (strain PAO1) biofilms after treating with SN from CV8 strains. CV8_D expresses payload D only; CV8_HA expresses payloads H and A. d Plot showing the quantification of biofilm dissolution by CV8 strains SN treatments in vitro, using the crystal violet assay. Data are shown as the mean of three independent experiments ± SEM. *** p < 0.001, ****p < 0.0001 by one-way Anova followed by the post-hoc Tukey’s multiple comparison tests.

    Article Snippet: The attenuated strain CV8 derives from the wild-type M. pneumoniae strain M129 (ATCC 29342, subtype 1), in which genes mpn133 and mpn372 were deleted, and the mpn051 gene was replaced by the gpsA gene from Mycoplasma penetrans .

    Techniques: Western Blot, Expressing, Dissolution, In Vitro, Crystal Violet Assay, Comparison